Reference library · Living world · Laboratory & sequence

DNA & microbial identification

A sequence match is a comparison between a query and a particular database release, not a species label delivered by a machine. The result depends on sample provenance, marker choice, read quality, trimming, alignment coverage, the quality of reference labels and whether the marker can separate the candidate taxa. These resources are selected because they expose reference accessions, vouchers, type strains, curation or release history that can travel with the claim.

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LIMITS
What this method can and cannot settle

Percentage identity alone never proves species. Short queries, conserved markers, contamination, mixed templates, sequencing errors and mislabelled references can all produce a persuasive wrong match. Report marker, primers, sequence quality, search service, database and release, alignment coverage, identity, gaps, top alternatives and reference provenance. A species determination needs a marker known to resolve that group plus agreement with morphology, ecology or voucher evidence; otherwise report the supported clade or operational unit.

Before you reach for an atlas

Record the characters first.

Matching a photograph against a memory is how misidentifications happen. Working through these in order gives you a description that someone else can check — and that stays useful even if the determination later changes.

  1. Sample and voucher

    Connect the sequence to a preserved voucher, culture, image or traceable source sample wherever possible.

    Record collector, date, place, tissue or substrate, extraction identifier and chain of custody. A sequence without a traceable source cannot repair a sample mix-up later.

  2. Marker and laboratory method

    Record locus, primers, extraction, amplification, library preparation and sequencing platform.

    State positive, negative and blank controls and any departures from the protocol. Different markers answer different taxonomic questions.

  3. Sequence quality

    Retain raw reads and report trimming, assembly, ambiguity, length and quality metrics.

    Do not submit only a consensus string. Preserve chromatograms or raw read files and the software and parameters that produced the query.

  4. Search and result

    Record database, release, algorithm, parameters, query date, coverage, identity, gaps and top alternative matches.

    A reproducible result needs accessions and versions. Look for type or voucher material and examine whether near-equal alternatives contradict a species-level claim.

  5. Agreement and uncertainty

    Compare the molecular result with morphology, ecology, geography and contamination controls.

    Report conflict rather than choosing the preferred answer. Operational clusters such as BINs and Species Hypotheses are useful units, not automatically formal species.

These are the observations. Standards covers the vocabularies that carry them into a record — including Darwin Core for biological occurrences, REMBI and OME for imaging, and ABCD-EFG for geological material — while each observation profile records its own method-specific context. Prepare a contribution covers what to keep alongside the files themselves.

Resources

Grouped by the question you are asking.

A resource can appear under more than one heading. Each entry states who maintains it, what it is best for, and where it stops being reliable.

Describe what you observed

Character guides and controlled terminology, so your description means the same thing to the next reader.

BacDive

Bacterial Diversity Metadatabase

Leibniz Institute DSMZ — German Collection of Microorganisms and Cell Cultures

Strain-level bacterial and archaeal taxonomy, morphology, physiology, cultivation, isolation, biosafety and sequence links, including type strains.

Best for
Checking whether a microbial candidate agrees with a documented strain's provenance, morphology, physiology and culture conditions.
Limits
Not a visual identification key. Culture-derived phenotypes can differ from environmental observations, uncultured diversity is underrepresented and record completeness varies.
Coverage
Global bacterial and archaeal strains held by culture collections and represented in the literature.
Reuse
CC BY 4.0 for BacDive data.
Contributing
Browsing is open; some programmatic services require free registration.
Database Open API Research pass ·

Compare against reference evidence

Atlases, catalogues and reference collections for comparing an unknown with documented material or measurements.

BOLD Systems

Barcode of Life Data Systems

Centre for Biodiversity Genomics, University of Guelph, with the International Barcode of Life community

A cross-taxon platform joining standard barcode sequences to specimen, voucher, image, collection and taxonomy metadata with identification engines.

Best for
Matching an appropriate barcode marker against specimen-linked references and inspecting the evidence behind the closest records.
Limits
Coverage and label quality are uneven, private records can make a result hard to reproduce, and Barcode Index Numbers are operational clusters rather than formally described species.
Coverage
Global animals, plants, fungi and other groups using supported barcode loci.
Reuse
Record, sequence and media rights vary by contributor and project; check every reference record before reuse.
Contributing
Public search and identification are free. An account is required for workbench, project and submission functions.
Database Open Research pass ·

NCBI BLAST

National Center for Biotechnology Information Basic Local Alignment Search Tool

National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health

General nucleotide and protein sequence-similarity searching across curated and broad public databases, with accession-level results.

Best for
Tracing a query to candidate accessions and publications when no more appropriate specialist identification service covers the marker.
Limits
Similarity is not identity. Broad databases contain submitter-supplied labels, contaminants and uneven curation, and no universal percentage threshold establishes a species.
Coverage
Global public nucleotide and protein sequence collections across all organisms.
Reuse
Public sequence records follow NCBI and source-database policies; cite accessions and the database searched.
Software or tool Open Research pass ·

SILVA

SILVA ribosomal RNA database

SILVA team at DSMZ Digital Diversity

Quality-controlled, aligned small- and large-subunit ribosomal RNA reference datasets with taxonomy, browsers and downloads.

Best for
Broad 16S, 18S, 23S or 28S placement using a release that can be recorded and reproduced.
Limits
Reference and Parc subsets have different curation levels; not every record is type-validated, eukaryotic curation is uneven and taxonomy changes between releases.
Coverage
Cellular life across bacterial, archaeal and eukaryotic ribosomal RNA loci.
Reuse
CC BY 4.0; cite SILVA and the exact release used.
Database Open Research pass ·

UNITE

UNITE community-governed collaboration centred on the University of Tartu

Curated fungal nuclear ITS references, expert annotations, reference sequences and DOI-bearing Species Hypotheses with analysis-ready releases.

Best for
Fungal ITS assignment with a versioned reference set and operational hypotheses that can be cited precisely.
Limits
Species Hypotheses are threshold-dependent clusters rather than formal species. ITS resolution differs among clades and underlying public labels can still be imperfect.
Coverage
Global fungi and selected other eukaryotes represented by ITS sequences.
Reuse
CC BY for UNITE releases; retain the release DOI and attribution.
Contributing
Browsing and downloads are open; registration is used for advanced PlutoF curation tools.
Database Open Research pass ·

PR²

Protist Ribosomal Reference database

International protist specialists coordinated through Station Biologique de Roscoff, CNRS and EukRef partners

An expert-curated 18S ribosomal RNA reference taxonomy for protists with downloadable releases and metabarcoding support.

Best for
Protist-specific 18S assignment using a curated taxonomic framework rather than a broad unfiltered sequence archive.
Limits
A sequence reference rather than morphology; 18S often cannot resolve species, environmental labels can be uncertain and expert coverage varies among clades.
Coverage
Global protists, with selected fungi, animals and plants retained for context.
Reuse
Open releases; cite PR² and record the database version used.
Database Open Research pass ·

BacDive

Bacterial Diversity Metadatabase

Leibniz Institute DSMZ — German Collection of Microorganisms and Cell Cultures

Strain-level bacterial and archaeal taxonomy, morphology, physiology, cultivation, isolation, biosafety and sequence links, including type strains.

Best for
Checking whether a microbial candidate agrees with a documented strain's provenance, morphology, physiology and culture conditions.
Limits
Not a visual identification key. Culture-derived phenotypes can differ from environmental observations, uncultured diversity is underrepresented and record completeness varies.
Coverage
Global bacterial and archaeal strains held by culture collections and represented in the literature.
Reuse
CC BY 4.0 for BacDive data.
Contributing
Browsing is open; some programmatic services require free registration.
Database Open API Research pass ·

TYGS

Type (Strain) Genome Server

Leibniz Institute DSMZ

Whole-genome identification and classification against type-strain genomes using digital DNA–DNA hybridisation, G+C comparison and phylogenomics.

Best for
Testing a bacterial or archaeal genome against type-strain references with a standards-oriented, documented workflow.
Limits
Requires a usable genome, can queue jobs and cannot compensate for missing or problematic type-genome data. Its result still needs sample and contamination scrutiny.
Coverage
Prokaryotic taxa with suitable type-strain genome references.
Reuse
DSMZ service terms apply; cite the TYGS method and references returned with the result.
Contributing
Submission is free and uses an email address to return results; no persistent user account is required.
Software or tool Free registration Research pass ·

Resolve the name or identifier

Authorities that settle an accepted biological or mineral name, astronomical designation or other stable identifier.

BOLD Systems

Barcode of Life Data Systems

Centre for Biodiversity Genomics, University of Guelph, with the International Barcode of Life community

A cross-taxon platform joining standard barcode sequences to specimen, voucher, image, collection and taxonomy metadata with identification engines.

Best for
Matching an appropriate barcode marker against specimen-linked references and inspecting the evidence behind the closest records.
Limits
Coverage and label quality are uneven, private records can make a result hard to reproduce, and Barcode Index Numbers are operational clusters rather than formally described species.
Coverage
Global animals, plants, fungi and other groups using supported barcode loci.
Reuse
Record, sequence and media rights vary by contributor and project; check every reference record before reuse.
Contributing
Public search and identification are free. An account is required for workbench, project and submission functions.
Database Open Research pass ·

NCBI BLAST

National Center for Biotechnology Information Basic Local Alignment Search Tool

National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health

General nucleotide and protein sequence-similarity searching across curated and broad public databases, with accession-level results.

Best for
Tracing a query to candidate accessions and publications when no more appropriate specialist identification service covers the marker.
Limits
Similarity is not identity. Broad databases contain submitter-supplied labels, contaminants and uneven curation, and no universal percentage threshold establishes a species.
Coverage
Global public nucleotide and protein sequence collections across all organisms.
Reuse
Public sequence records follow NCBI and source-database policies; cite accessions and the database searched.
Software or tool Open Research pass ·

SILVA

SILVA ribosomal RNA database

SILVA team at DSMZ Digital Diversity

Quality-controlled, aligned small- and large-subunit ribosomal RNA reference datasets with taxonomy, browsers and downloads.

Best for
Broad 16S, 18S, 23S or 28S placement using a release that can be recorded and reproduced.
Limits
Reference and Parc subsets have different curation levels; not every record is type-validated, eukaryotic curation is uneven and taxonomy changes between releases.
Coverage
Cellular life across bacterial, archaeal and eukaryotic ribosomal RNA loci.
Reuse
CC BY 4.0; cite SILVA and the exact release used.
Database Open Research pass ·

UNITE

UNITE community-governed collaboration centred on the University of Tartu

Curated fungal nuclear ITS references, expert annotations, reference sequences and DOI-bearing Species Hypotheses with analysis-ready releases.

Best for
Fungal ITS assignment with a versioned reference set and operational hypotheses that can be cited precisely.
Limits
Species Hypotheses are threshold-dependent clusters rather than formal species. ITS resolution differs among clades and underlying public labels can still be imperfect.
Coverage
Global fungi and selected other eukaryotes represented by ITS sequences.
Reuse
CC BY for UNITE releases; retain the release DOI and attribution.
Contributing
Browsing and downloads are open; registration is used for advanced PlutoF curation tools.
Database Open Research pass ·

PR²

Protist Ribosomal Reference database

International protist specialists coordinated through Station Biologique de Roscoff, CNRS and EukRef partners

An expert-curated 18S ribosomal RNA reference taxonomy for protists with downloadable releases and metabarcoding support.

Best for
Protist-specific 18S assignment using a curated taxonomic framework rather than a broad unfiltered sequence archive.
Limits
A sequence reference rather than morphology; 18S often cannot resolve species, environmental labels can be uncertain and expert coverage varies among clades.
Coverage
Global protists, with selected fungi, animals and plants retained for context.
Reuse
Open releases; cite PR² and record the database version used.
Database Open Research pass ·

BacDive

Bacterial Diversity Metadatabase

Leibniz Institute DSMZ — German Collection of Microorganisms and Cell Cultures

Strain-level bacterial and archaeal taxonomy, morphology, physiology, cultivation, isolation, biosafety and sequence links, including type strains.

Best for
Checking whether a microbial candidate agrees with a documented strain's provenance, morphology, physiology and culture conditions.
Limits
Not a visual identification key. Culture-derived phenotypes can differ from environmental observations, uncultured diversity is underrepresented and record completeness varies.
Coverage
Global bacterial and archaeal strains held by culture collections and represented in the literature.
Reuse
CC BY 4.0 for BacDive data.
Contributing
Browsing is open; some programmatic services require free registration.
Database Open API Research pass ·

LPSN

List of Prokaryotic names with Standing in Nomenclature

DSMZ Digital Diversity

A reference for valid publication status, nomenclatural history, synonyms, type strains and literature under the prokaryotic nomenclature code.

Best for
Checking whether a bacterial or archaeal name is validly published and identifying its nomenclatural type strain.
Limits
Nomenclature is not identification and LPSN is not an official universal biological classification. A valid name says nothing about whether the query belongs to that taxon.
Coverage
Global names of Bacteria and Archaea governed by the International Code of Nomenclature of Prokaryotes.
Reuse
Attribution and DSMZ Digital Diversity terms apply; API and downloads require registration.
Taxonomic authority Open Research pass ·

TYGS

Type (Strain) Genome Server

Leibniz Institute DSMZ

Whole-genome identification and classification against type-strain genomes using digital DNA–DNA hybridisation, G+C comparison and phylogenomics.

Best for
Testing a bacterial or archaeal genome against type-strain references with a standards-oriented, documented workflow.
Limits
Requires a usable genome, can queue jobs and cannot compensate for missing or problematic type-genome data. Its result still needs sample and contamination scrutiny.
Coverage
Prokaryotic taxa with suitable type-strain genome references.
Reuse
DSMZ service terms apply; cite the TYGS method and references returned with the result.
Contributing
Submission is free and uses an email address to return results; no persistent user account is required.
Software or tool Free registration Research pass ·

Place it in context

Occurrence, stratigraphic, catalogue and archive services that situate a record in space, time and literature.

BOLD Systems

Barcode of Life Data Systems

Centre for Biodiversity Genomics, University of Guelph, with the International Barcode of Life community

A cross-taxon platform joining standard barcode sequences to specimen, voucher, image, collection and taxonomy metadata with identification engines.

Best for
Matching an appropriate barcode marker against specimen-linked references and inspecting the evidence behind the closest records.
Limits
Coverage and label quality are uneven, private records can make a result hard to reproduce, and Barcode Index Numbers are operational clusters rather than formally described species.
Coverage
Global animals, plants, fungi and other groups using supported barcode loci.
Reuse
Record, sequence and media rights vary by contributor and project; check every reference record before reuse.
Contributing
Public search and identification are free. An account is required for workbench, project and submission functions.
Database Open Research pass ·

NCBI BLAST

National Center for Biotechnology Information Basic Local Alignment Search Tool

National Center for Biotechnology Information, U.S. National Library of Medicine, National Institutes of Health

General nucleotide and protein sequence-similarity searching across curated and broad public databases, with accession-level results.

Best for
Tracing a query to candidate accessions and publications when no more appropriate specialist identification service covers the marker.
Limits
Similarity is not identity. Broad databases contain submitter-supplied labels, contaminants and uneven curation, and no universal percentage threshold establishes a species.
Coverage
Global public nucleotide and protein sequence collections across all organisms.
Reuse
Public sequence records follow NCBI and source-database policies; cite accessions and the database searched.
Software or tool Open Research pass ·

SILVA

SILVA ribosomal RNA database

SILVA team at DSMZ Digital Diversity

Quality-controlled, aligned small- and large-subunit ribosomal RNA reference datasets with taxonomy, browsers and downloads.

Best for
Broad 16S, 18S, 23S or 28S placement using a release that can be recorded and reproduced.
Limits
Reference and Parc subsets have different curation levels; not every record is type-validated, eukaryotic curation is uneven and taxonomy changes between releases.
Coverage
Cellular life across bacterial, archaeal and eukaryotic ribosomal RNA loci.
Reuse
CC BY 4.0; cite SILVA and the exact release used.
Database Open Research pass ·

UNITE

UNITE community-governed collaboration centred on the University of Tartu

Curated fungal nuclear ITS references, expert annotations, reference sequences and DOI-bearing Species Hypotheses with analysis-ready releases.

Best for
Fungal ITS assignment with a versioned reference set and operational hypotheses that can be cited precisely.
Limits
Species Hypotheses are threshold-dependent clusters rather than formal species. ITS resolution differs among clades and underlying public labels can still be imperfect.
Coverage
Global fungi and selected other eukaryotes represented by ITS sequences.
Reuse
CC BY for UNITE releases; retain the release DOI and attribution.
Contributing
Browsing and downloads are open; registration is used for advanced PlutoF curation tools.
Database Open Research pass ·

PR²

Protist Ribosomal Reference database

International protist specialists coordinated through Station Biologique de Roscoff, CNRS and EukRef partners

An expert-curated 18S ribosomal RNA reference taxonomy for protists with downloadable releases and metabarcoding support.

Best for
Protist-specific 18S assignment using a curated taxonomic framework rather than a broad unfiltered sequence archive.
Limits
A sequence reference rather than morphology; 18S often cannot resolve species, environmental labels can be uncertain and expert coverage varies among clades.
Coverage
Global protists, with selected fungi, animals and plants retained for context.
Reuse
Open releases; cite PR² and record the database version used.
Database Open Research pass ·

BacDive

Bacterial Diversity Metadatabase

Leibniz Institute DSMZ — German Collection of Microorganisms and Cell Cultures

Strain-level bacterial and archaeal taxonomy, morphology, physiology, cultivation, isolation, biosafety and sequence links, including type strains.

Best for
Checking whether a microbial candidate agrees with a documented strain's provenance, morphology, physiology and culture conditions.
Limits
Not a visual identification key. Culture-derived phenotypes can differ from environmental observations, uncultured diversity is underrepresented and record completeness varies.
Coverage
Global bacterial and archaeal strains held by culture collections and represented in the literature.
Reuse
CC BY 4.0 for BacDive data.
Contributing
Browsing is open; some programmatic services require free registration.
Database Open API Research pass ·

LPSN

List of Prokaryotic names with Standing in Nomenclature

DSMZ Digital Diversity

A reference for valid publication status, nomenclatural history, synonyms, type strains and literature under the prokaryotic nomenclature code.

Best for
Checking whether a bacterial or archaeal name is validly published and identifying its nomenclatural type strain.
Limits
Nomenclature is not identification and LPSN is not an official universal biological classification. A valid name says nothing about whether the query belongs to that taxon.
Coverage
Global names of Bacteria and Archaea governed by the International Code of Nomenclature of Prokaryotes.
Reuse
Attribution and DSMZ Digital Diversity terms apply; API and downloads require registration.
Taxonomic authority Open Research pass ·